| HPLC法测定脂质体松萝酸中松萝酸的含量 |
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| 引用本文： | 李继,于吉臣,傅经国,陈涛,王晓来. HPLC法测定脂质体松萝酸中松萝酸的含量[J]. 分析试验室, 2010, 29(2) |
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| 作者姓名： | 李继 于吉臣 傅经国 陈涛 王晓来 |
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| 作者单位： | 中国科学院兰州化学物理研究所研究生院,兰州,730002;陕西省脂质体工程技术研究中心,西安,710075 |
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| 摘 要： | 建立了HPLC测定脂质体松萝酸中药物松萝酸的方法。以Kromasil C18反相色谱柱(250 mmⅹ4.6 mm,5μm)为分离柱,流动相组成为V(甲醇)∶V(磷酸盐缓冲液(pH 5.0))=7∶3,流速1 mL/min,紫外检测,波长284 nm。松萝酸在10~50μg/mL的范围内有很好的线性关系(r=0.9994)。加样回收率为100.1%±0.6%,RSD为0.54%±0.07%。本方法适用于脂质体松萝酸中药物松萝酸的测定。
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| 关 键 词： | 松萝酸 高效液相色谱 脂质体松萝酸 |
HPLC determination of usnic acid in liposomal usnic acid formulations |
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| LI Ji,YU Ji-chen,FU Jing-guo,CHEN Tao,WANG Xiao-lai. HPLC determination of usnic acid in liposomal usnic acid formulations[J]. Chinese Journal of Analysis Laboratory, 2010, 29(2) |
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| Authors: | LI Ji YU Ji-chen FU Jing-guo CHEN Tao WANG Xiao-lai |
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| Abstract: | To establish a HPLC method for the detection of usnic acid in liposomal usnic acid. Kromasil C18 column (250 mm×4.6 mm, 5 μm) was used, and 1.0 mL/min and the determination wavelength was at 284 nm. The linear range was in the range of 10～50 μg/mL (r=0.9994). The average recovery was 100.1%±0.6%;RSD was 0.54%±0.07%. This method is simple, rapid and reliable for the determination of usnic acid in liposomal usnic acid formulations. |
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| Keywords: | Usnic acid HPLC Liposomal usnic acid |
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