The gold-nanoparticle-based surface plasmon resonance light scattering and visual DNA aptasensor for lysozyme |
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Authors: | Xinyi Wang Yao Xu Yang Chen Limei Li Feng Liu Na Li |
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Institution: | (1) Beijing National Laboratory for Molecular Sciences (BNLMS), The Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China;(2) College of Sciences, Shenyang Agricultural University, Shenyang, 110161, China; |
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Abstract: | We developed a new simple and sensitive assay for lysozyme based on gold nanoparticle plasmon resonance light scattering (PRLS)
measurement and naked-eye detection using for the first time the lysozyme DNA aptamer as the recognition element. Lysozyme
DNA aptamer could stabilize gold nanoparticles (AuNPs) at high ionic strength. Introducing lysozyme to the system easily triggered
the aggregation of AuNPs, producing a red-to-blue color change of the solution, red-shifted plasmon absorption, and enhanced
plasmon resonance light scattering. The linear range was found to be 0.2∼4 nM for 0.7 nM AuNPs, 0.3∼6 nM for 1.4 nM AuNPs
and 0.6∼8 nM for 2.1 nM AuNPs. About 0.1 nM lysozyme can produce an observable enhancement of PRLS signal. For visual detection,
1 nM lysozyme can produce a very distinctive color change. Satisfactory recoveries were obtained for simulated saliva and
diluted urine samples, indicating that the method has potential for analyses of clinical samples. The simplicity and high
sensitivity that are consistent with the resources and needs of many laboratories makes this method a good choice for routine
analysis. |
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