| 基于苯并吡喃腈-香豆素体系的近红外比率型荧光探针用于检测He La细胞中的过氧亚硝酸盐(英文) |
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| 引用本文： | 陈宇,毕克英,郝瑞亭,谢萍,黄丽娜,王玉敏,张俊峰,徐锐,吴相华. 基于苯并吡喃腈-香豆素体系的近红外比率型荧光探针用于检测He La细胞中的过氧亚硝酸盐(英文)[J]. 有机化学, 2020, 0(2): 511-515 |
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| 作者姓名： | 陈宇 毕克英 郝瑞亭 谢萍 黄丽娜 王玉敏 张俊峰 徐锐 吴相华 |
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| 作者单位： | 云南师范大学化学化工学院;云南师范大学能源与环境科学学院 |
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| 基金项目： | 国家自然科学基金(Nos.21262050,21302165,21672185,21867019,41561108,61774130,11474248);云南省科学技术厅基金(Nos.2016FB020,2014HB008,2017HB019);云南师范大学研究生科研创新基金(No.Ysdyjs2019122)资助项目。 |
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| 摘 要： | 过氧亚硝酸根(ONOO-)是生物体内的一种重要的活性氧,与人体内的各种生理活动息息相关.利用苯并吡喃腈-香豆素体系,合成了一种用于ONOO-检测的近红外比率型荧光探针(E)-7-(二乙胺基)-3-(2-(苯并吡喃腈)乙烯基)-香豆素(DCCM).该探针在ONOO-存在下表现出强烈的响应,颜色从紫色变为浅粉色,最大发射峰蓝移217nm,荧光颜色从淡紫色变为蓝色,能够直观地对溶液中的ONOO-进行监测. DCCM可以灵敏地检测ONOO^-,最低检测限为6.0×10^-7 mol·L^-1,该探针被成功用于HeLa细胞中的ONOO-成像检测.
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| 关 键 词： | 荧光探针 比率探针 过氧亚硝酸盐 细胞成像 |
Near-Infrared Ratiometric Fluorescent Probe for Detection of Peroxynitrite in He La Cells Based on Dicyanomethylene-4H-pyran Coumarin System |
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| Chen Yu,Bi Keying,Hao Ruiting,Xie Ping,Huang Lina,Wang Yumin,Zhang Junfeng,Xu Rui,Wu Xianghua. Near-Infrared Ratiometric Fluorescent Probe for Detection of Peroxynitrite in He La Cells Based on Dicyanomethylene-4H-pyran Coumarin System[J]. Chinese Journal of Organic Chemistry, 2020, 0(2): 511-515 |
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| Authors: | Chen Yu Bi Keying Hao Ruiting Xie Ping Huang Lina Wang Yumin Zhang Junfeng Xu Rui Wu Xianghua |
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| Affiliation: | (College of Chemistry and Chemical Engineering,Yunnan Normal University,Kunming 650500;College of Energy and Environment Science,Yunnan Normal University,Kunming 650500) |
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| Abstract: | Peroxynitrite(ONOO-) is an important reactive oxygen species in living organisms, and is associated with various biological process in human body. Using dicyanomethylene-4 H-pyran coumarin system, a near-infrared ratiometric fluorescent probe(E)-7-(diethylamino)-3-(2-(dicyanomethylene-4 H-pyran)vinyl)-coumarin(DCCM) was developed for ONOO-detection. This probe exhibited a strong response in the presence of ONOO^-, with a significant color change from purple to light pink, along with 217 nm fluorescence blue shift. The emission color changed from heliotrope to blue, which enables the monitoring of ONOO-by naked eyes. DCCM was used to sensitively measure ONOO-with low detection limit of 6.0×10^-7 mol·L^-1. The use of DCCM for imaging ONOO-in HeLa cells was also demonstrated. |
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| Keywords: | fluorescence probe ratiometric probe peroxynitrite cell imaging |
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