Dissociation and fractionation of heavy and light chains from IgG monoclonal antibodies |
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Authors: | Gagnon Pete Rodriquez George Zaidi Simin |
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Affiliation: | Bioprocessing Technology Institute, 20 Biopolis Way, Centros, Singapore. pete_gagnon@mac.com |
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Abstract: | A basic method for dissociation and fractionation of monoclonal IgG heavy and light chain is described. It employs less noxious and hazardous reagents than the classical mercaptoethanol/propionic acid process and replaces size exclusion chromatography with cation exchange on a monolith to improve productivity. Significant scope remains to refine the conditions. The method can be applied to other disulfide bonded proteins with significant affinity for cation exchangers. |
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Keywords: | IgG Heavy chain Light chain Fractionation Monoliths |
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