Rapid Screening Alpha-Glucosidase Inhibitors from Polygoni Vivipari Rhizoma by Multi-Step Matrix Solid-Phase Dispersion,Ultrafiltration and HPLC |
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Authors: | Haoxiang Li Zhuobin He Qianhui Shen Weifeng Fan Guoying Tan Yuansheng Zou Quanxi Mei Zhengming Qian |
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Affiliation: | 1.Studio of National Chinese Medical Science Master Shiyuan Jin, Bao’an Authentic TCM Therapy Hospital, Shenzhen 518101, China;2.Key Laboratory of State Administration of Traditional Chinese Medicine, Dongguan HEC Cordyceps R&D Co., Ltd., Dongguan 523850, China; (Z.H.); (Q.S.); (W.F.); (G.T.); (Y.Z.) |
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Abstract: | Polygoni Vivipari Rhizoma (PVR), the dried root of Polygonum viviparum, has been used as herbal medicine in China for a long time. In the present study, a new method based on multi-step matrix solid-phase dispersion (MSPD), ultrafiltration and high performance liquid chromatography (HPLC) for screening alpha-glucosidase inhibitors (AGIs) from PVR was proposed. First, three different PVR extractions were prepared by multi-step MSPD with 15% methanol, 60% methanol and 100% methanol. Second, the alpha-glucosidase inhibition tests for the three extracts were carried out, and the 60% methanol extraction showed the best activity. Then, the AGIs screening experiment was performed with ultrafiltration and HPLC analysis using the 60% methanol extraction. Seven binding components (quercetin−3−O−vicianoside, quercetin 3−O−neohesperidoside, rutin, hyperoside, quercetin 3−O−glucuronide, luteolin−7−O−neohesperidoside, kaempferol 3−glucuronide) were found. These seven components were further validated as the AGIs by molecular docking analysis. The developed method was a rapid and efficient tool for screening AGIs from PVR, which provided scientific data for the bioactive components study of PVR. |
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Keywords: | Polygoni Vivipari Rhizoma alpha-glucosidase inhibitors MSPD ultrafiltration HPLC |
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