Analysis of the Composition of Deinagkistrodon acutus Snake Venom Based on Proteomics,and Its Antithrombotic Activity and Toxicity Studies |
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| Authors: | Jin Huang Minrui Zhao Chu Xue Jiqiang Liang Fang Huang |
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| Affiliation: | 1.Shenzhen Pingle Orthopedic Hospital (Shenzhen Pingshan Traditional Chinese Medicine Hospital), Shenzhen 518001, China; (J.H.); (M.Z.);2.Jiangsu Key Laboratory of TCM Evaluation and Translational Research, Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 639 Longmian Road, Nanjing 211198, China; |
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| Abstract: | There is a strong correlation between the composition of Deinagkistrodon acutus venom proteins and their potential pharmacological effects. The proteomic analysis revealed 103 proteins identified through label-free proteomics from 30 different snake venom families. Phospholipase A2 (30.0%), snaclec (21.0%), antithrombin (17.8%), thrombin (8.1%) and metalloproteinases (4.2%) were the most abundant proteins. The main toxicity of Deinagkistrodon acutus venom is hematotoxicity and neurotoxicity, and it acts on the lung. Deinagkistrodon acutus venom may have anticoagulant and antithrombotic effects. In summary, the protein profile and related toxicity and pharmacological activity of Deinagkistrodon acutus venom from southwest China were put forward for the first time. In addition, we revealed the relationship between the main toxicity, pharmacological effects, and the protein components of snake venom. |
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| Keywords: | Deinagkistrodon acutus venom proteomic analysis label-free proteomics hematotoxicity antithrombosis anticoagulation |
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