Separation,Purification, Structural Characterization,and Anticancer Activity of a Novel Exopolysaccharide from Mucor sp. |
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| Authors: | Jiaojiao Wang Pingchuan Yuan Wenzhi Zhang Chunyan Liu Kaoshan Chen Guodong Wang Taili Shao |
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| Institution: | 1.Drug Research & Development Center, School of Pharmacy, Wannan Medical College, Wuhu 241002, China; (J.W.); (P.Y.); (W.Z.); (C.L.);2.Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Provincial Engineering Laboratory for Screening and Re-Evaluation of Active Compounds of Herbal Medicines in Southern Anhui, Wuhu 241002, China;3.School of Life Science, Shandong University, Jinan 250100, China |
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| Abstract: | Mucor sp. has a wide range of applications in the food fermentation industry. In this study, a novel exopolysaccharide, labeled MSEPS, was separated from Mucor sp. fermentation broth through ethanol precipitation and was purified by ion-exchange chromatography, as well as gel filtration column chromatography. MSEPS was composed mostly of mannose, galactose, fucose, arabinose, and glucose with a molar ratio of 0.466:0.169:0.139:0.126:0.015 and had a molecular weight of 7.78 × 104 Da. The analysis of methylation and nuclear magnetic resonance results indicated that MSEPS mainly consisted of a backbone of →3,6)-α-d-Manp-(1→3,6)-β-d-Galp-(1→, with substitution at O-3 of →6)-α-d-Manp-(1→ and →6)-β-d-Galp-(1→ by terminal α-l-Araf residues. MTT assays showed that MSEPS was nontoxic in normal cells (HK-2 cells) and inhibited the proliferation of carcinoma cells (SGC-7901 cells). Additionally, morphological analysis and flow cytometry experiments indicated that MSEPS promoted SGC-7901 cell death via apoptosis. Therefore, MSEPS from Mucor sp. can be developed as a potential antitumor agent. |
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| Keywords: | exopolysaccharide from Mucor sp structural characterization anticancer activity |
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