固定化过氧化氢酶的制备及其抗氧化作用

以烟用醋酸纤维的生物化学改性为目标,研究了以壳聚糖为载体时,吸附交联固定化过氧化氢酶的条件,并考察了固定化酶的性质。结果表明,固定化的最佳条件为:加酶量(酶活2×104C IU/m l)6m l,3%壳聚糖20m l,乙二醛浓度6%(w/v),交联剂用量100m l,吸附时间0.5 h,交联时间2.5h,酶活收率可达42.9%。过氧化氢酶固定化后,动学参数Km值为61.7mmol/L;对活性氧具有较好清除作用。

Preparation of immobilized catalase and its anti-oxidation

The biochemical modification of cellulose acetate fiber used in cigarette was investigated in this study.Catalase was immobilized on chitosan with glyoxal by cross-linking reaction.The immobilization conditions was studied as well as characteristics of immobilized catalase.The optimal conditions for immobilization were as follows:3% chitosan 20ml,30%glyozal 10ml,liquid enzyme 6ml;the concentration of gryoxal was 6%,the optimal reaction time was 0.5h.The activity yield of the immobilized catalase was 42.9% as the orinal.The characteristics of the immobilized catalase such as the optimal pH and temperature range,thermal stability,and storage stability were also assessed.The K_m,an important kinetic parameter of enzyme,was obtained as 61.7 mmol/L with Lineweaver-bulk method.Moreover,the immobilized catalase was found that it removed superoxide anion free radical(SAFR) produced in pyrogallic acid system.