| 毛细管区带电泳激光诱导荧光间接检测环腺苷单磷酸和环鸟苷单磷酸 |
| |
| 引用本文： | 陈勇,杨新,韩凤梅,袁倬斌.毛细管区带电泳激光诱导荧光间接检测环腺苷单磷酸和环鸟苷单磷酸[J].分析化学,1999,27(6):694-696. |
| |
| 作者姓名： | 陈勇 杨新 韩凤梅 袁倬斌 |
| |
| 作者单位： | 1. 湖北大学生命科学学院,武汉,430062
2. 中国科学技术大学研究生院,北京,100039 |
| |
| 基金项目： | 国家自然科学基金，武汉科委“晨光计划”资助课题 |
| |
| 摘 要： | 研究了影响毛细管区带电泳激光诱导荧光间接检测环腺苷单磷酸和环鸟苷单磷酸的实验条件.cAMP和cGMP的线性范围分别为30～500mg/L和15～500mg/L,其最低检测限分别为9.0mg/L和0.5mg/L;当浓度为125mg/L时,cAMP和cGMP峰面积的RSD为4.45%和6.21%(n=4);其迁移时间的日间RSD分别为2.92%和2.04%(n=5)
|
| 关 键 词： | 毛细管区带电泳 激光诱导荧光间接检测 环腺单磷酸 环鸟单磷酸 |
| 修稿时间： | 1998年7月13日 |
Separation of Cyclic-adenosine monophosphate(cAMP) and Cyclic-guanosine monophosphate(cGMP) by Capillary Zone Electrophoresis With Indirect Laser-induced Fluorescence Detection |
| |
| Chen Yong,Yang Xin,Han Fengmei,Yuan Zhuobin.Separation of Cyclic-adenosine monophosphate(cAMP) and Cyclic-guanosine monophosphate(cGMP) by Capillary Zone Electrophoresis With Indirect Laser-induced Fluorescence Detection[J].Chinese Journal of Analytical Chemistry,1999,27(6):694-696. |
| |
| Authors: | Chen Yong Yang Xin Han Fengmei Yuan Zhuobin |
| |
| Abstract: | This paper reports the optimum conditions of separation and determination of cyclic-adenosine monophosphate( cAMP ) and cyclic-guanosine monophosphate(cGMP) by capillary zone electrophoresis with indirect laser-induced fluorescence detection. The linear ranges of determination for cAMP and cGMP are 30-500 mg/L and 15-500 mg/L. The concentration limit of detection ( CLOD) for cAMP and cGMP are 9.0 mg/L and 0.5 mg/L. The RSD of peak areas of cAMP and cGMP are 4.5% and 6.2% ( n - 4) in the 125 mg/L; the intra-day RSD of the retention times of cAMP and cGMP are 2.9% and 2. 0% ( n = 5) , respectively. |
| |
| Keywords: | Capillary zone electrophoresis indirect laser-induced fluorescence detection cyclic-adenosine monophosphate cyclic-guanosine monophosphate |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
