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Chemical composition and physicochemical characteristics of fixed oils from algerian <Emphasis Type="Italic">Nigella sativa</Emphasis> seeds
Authors:Farid Benkaci-Ali  Aoumeur Baaliouamer  Jean Paul Wathelet  Michel Marlier
Institution:(1) Universite des Sciences et de la Technologie Houari Boumediene U.S.T.H.B, Faculte de Chimie, Laboratoire d’Analyse Organique Fonctionnelle BP. 32 El Alia, Bab Ezzouar, Algerie;(2) Centre de Recherche Scientifique et Technique en Analyses Physico-Chimiques C.R.A.P.C, B. P. 248 RP 16004 Alger, Algerie;(3) Faculte Universitaire des Sciences Agronomiques de Gembloux, Laboratoire de Chimie Generale, Passage des Deportes 2 B-5030, Gembloux, Belgique
Abstract:The fatty acids, sterols, and polyphenols from the fixed oils of Nigella sativa seeds originating from four locations were determined. The seeds contained respectively 30.63–34.27% and 25.66–32.77% of fixed oils using hexane and isopropyl alcohol in solvent extraction. Linoleic, oleic, and palmitic acids formed the main proportion using the two solvents, respectively: hexane 54.47–61.28%, isopropanol 56.98–67.30%; hexane 19.62–22.94%, isopropanol 18.85–21.96%, and hexane 11.17–13.60%, isopropanol 9.20–14.18%. Other minor unsaturated fatty acids were identified. Eight phytosterols were isolated and identified in the fixed oils by GC and GC/MS analysis, wherein β-sitosterol was the dominating compound that inhibits the absorption of dietary cholesterol, followed by stigmasterol, campesterol, and Δ5-avenasterol.
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