Structure-guided redesign of D-fructose-6-phosphate aldolase from E. coli: remarkable activity and selectivity towards acceptor substrates by two-point mutation |
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| Authors: | Gutierrez Mariana Parella Teodor Joglar Jesús Bujons Jordi Clapés Pere |
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| Institution: | Instituto de Química Avanzada de Catalu?a-CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain. |
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| Abstract: | Structure-guided re-design of the acceptor binding site of D-fructose-6-phosphate aldolase from E. coli leads to the construction of FSA A129S/A165G double mutant with an activity between 5- to >900-fold higher than that of wild-type towards N-Cbz-aminoaldehyde derivatives. |
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