高效液相色谱法测定烤肉中的苯并(a)芘

建立了测定烤肉中苯并(α)芘含量的高效液相色谱法。采用CNW~Athena C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇–水(90∶10),流量为1.0 m L/min,柱温为35℃,进样体积为10μL,荧光检测器激发波长为365 nm,发射波长为410 nm,以色谱峰面积标准曲线法定量。苯并(α)芘的质量浓度在0~0.2μg/m L范围内与色谱峰面积呈良好的线性关系,相关系数为0.999 5,检出限为0.07μg/kg,加标回收率为91.3%~92.6%,测定结果的相对标准偏差为1.21%~1.40%(n=5)。该法样品预处理简便,检测时间短,灵敏度高,适用于烤肉中苯并(α)芘的含量测定。

Determination of Benzo(a)pyrene Content in Barbecue by High Performance Liquid Chromatography

HPLC method for determining the content of benzo(a)pyrene in barbecue was set up with CNW? Athena C18 column (250 mm×4.6 mm, 5μm), methanol-water (90∶10) as the mobile phase, the flow rate of 1.0 mL/min, the column temperature of 35℃, the injection volume of 10μL, the excitation wavelength of 365 nm, the emission wavelength of 410 nm, and the standard curve method for quantitative analysis by peak area. The concentration of benzo(a)-pyrene in barbecue showed a good linearity with the chromatography peak area in the range of 0–0.2μg/mL with the correlation of 0.999 5, and the limit of quantity of the method was 0.07μg/kg. The recoveries of benzo(a)pyrene were 91.3%–92.6%, and the relative standard deviation of the determination results was 1.21%–1.40%(n=5). This method has advantages of simple process of sample treatment, short analysis time, and high sensity,and it is suitable for the qualitative determination of benzo(a)pyrene in barbecue.