Entrapment of invertase in an interpenetrated polymer network of alginic acid and poly (1-vinylimidazole) |
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| Authors: | Mü jgan Kartal,Ayhan Bozkurt |
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| Affiliation: | a Department of Chemistry, Middle East Technical University, 06531 Ankara, Turkey
b Department of Chemistry, Fatih University, 34900 Büyükçekmece, Istanbul, Turkey |
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| Abstract: | In this work, we have investigated the synthesis and characterization of a proton conductor based on alginic acid and poly (1-vinylimidazole). The polymer network was obtained by mixing alginic acid and poly (1-vinylimidazole) at various stoichiometric ratios. The polymer electrolytes were characterized by elemental analysis and FT-IR spectroscopy. Invertase was entrapped in the polymer networks during complex formation. Additionally, the maximum reaction rate and Michaelis-Menten constant were investigated for the immobilized invertase. The temperature and pH optimization, operational stability and shelf life of the polymer network were examined. |
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| Keywords: | Alginic acid Poly (1-vinylimidazole) Invertase Enzyme immobilization Proton conductivity |
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