| 秀丽小杆线虫Caenorhabditis elegans pkc-2基因cDNA的克隆和表达 |
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| 引用本文： | 钱雨,Laurent SGALAT.秀丽小杆线虫Caenorhabditis elegans pkc-2基因cDNA的克隆和表达[J].华东师范大学学报(自然科学版),2009,2009(6):134-140. |
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| 作者姓名： | 钱雨 Laurent SGALAT |
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| 作者单位： | 1. 华东师范大学 生命科学学院, 上海200062; 2. 克洛德•贝纳尔-里昂第一大学 细胞与分子遗传学中心, 法国 里昂69100 |
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| 摘 要： | 蛋白激酶C在秀丽小杆线虫中具有调节肌细胞渐进性萎缩的功能.为了揭示它的调节机制, 本研究克隆了秀丽小杆线虫中蛋白激酶C pkc-2基因的cDNA pkc-2-c，构建了含该pkc-2基因cDNA亚型的重组质粒pPD 118.20-pKG 63；揭示了该cDNA在秀丽小杆线虫体壁肌细胞中的定位.
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| 关 键 词： | 秀丽小杆线虫 pkc-2基因 cDNA克隆 免疫细胞化学 秀丽小杆线虫 pkc-2基因 cDNA克隆 免疫细胞化学 |
| 收稿时间： | 2008-10-10 |
| 修稿时间： | 2008-11-27 |
Cloning and expression pattern analysis of a cDNA of pkc-2gene in Caenorhabditis elegans (Chinese, Notes) |
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| QIAN Yu,Laurent SGALAT.Cloning and expression pattern analysis of a cDNA of pkc-2gene in Caenorhabditis elegans (Chinese, Notes)[J].Journal of East China Normal University(Natural Science),2009,2009(6):134-140. |
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| Authors: | QIAN Yu Laurent SGALAT |
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| Institution: | 1. School of Life Science， East China Normal University, Shanghai200062, China; 2. Center for Cell and Molecular Genetics, Université Claude Bernard Lyon 1, Lyon 69100, France |
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| Abstract: | Protein kinase C regulates the progressive muscle degeneration in Caenorhabditis elegans. In order to investigate the function of PKC involved in muscle degeneration, this paper cloned a cDNA isoform of pkc-2 gene of C.elegans and constructed the recombinant plasmids pPD118.20-pKG63 containing the isoform. The new isoform was then further studied for gene expression pattern. Immunocytochemistry experiment showed that this cDNA isoform expressed in body-wall muscle cells and located near the dense body. |
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| Keywords: | Caenorhabditis elegans pkc-2 gene clone immunocytochemistry |
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