聚乙二醇-硫酸铵双水相萃取次甲基蓝的研究

利用分光光度法研究了次甲基蓝在聚乙二醇-硫酸铵双水相体系的萃取行为,探索了质量配比(mPEc/m(NH4)2SO4)、温度和次甲基蓝的浓度对双水相及次甲基蓝萃取率(Y)的影响.实验表明:(1)在一定的温度和浓度下,随着mPEC/m(NH4)2SO4的减小,分配系数K( cup/clow)增加,萃取率Y略有减小；(2)在一定的质量配比和浓度下,分配系数K(cup/clow)随温度的升高而增大,但萃取率Y却基本保持不变；(3)在一定的质量配比和温度下,随着次甲基蓝浓度的增加,分配系数K(cup/clow)增大；萃取率Y略有增加.在mPEG/m( NH4) 2SO4为1.50∶1,c次甲基蓝=8.915×10-5mol/L、t=30℃的实验条件下,K(cup/clow)=22.14,萃取率(y)可达95.43％.

Study on extraction of methylene blue in PEG/ammonium sulfate two-phase aqueous systems

Extraction behaviors of Methylene Blue in PEG /ammonium sulfate aqueous two-phase systems were studied by Spectrophotometry.Effects of mass ratio(m PEG / m(NH4) 2SO4) 、temperature and concentration of Crystal violet on the aqueous two-phase systems and extraction yield(Y) were investigated.The experiments results show:(1) the partition ratio K(c up / c low) was increased,but the extraction yield(Y) was decreased slightly with m PEG / m(NH4) 2SO4 decreasing under a certain temperature and concentration;(2) under a certain m PEG / m(NH4) 2SO4 and concentration partition ratio K(c up / c low) for Crystal violet was obviously increased with temperature increasing,and extraction yield(Y) was nearly unchanged;(3) under a certain m PEG / m(NH4) 2SO4 and temperature the partition ratio K(c up / c low) was increased with concentration of Crystal violet increasing,but extraction yield(Y) was increased slightly.the extraction yield(Y) reaches to 95.43% when m PEG / m(NH4) 2SO4 = 1.50 ∶1、t = 30℃ and cMethylene Blue = 8.915×10-5 mol / L.