红花黄色素抗氧化活性研究

通过考察红花黄色素(SY)及其主要化学成分对羟基自由基介导2-脱氧核糖氧化降解的抑制作用,以及对1,1-二苯基-2-苦肼基自由基(DPPH·)的清除能力,探究SY的体外抗氧化活性及其抗氧化的主要有效成分.通过Fenton反应产生羟基自由基,用紫外分光光度计检测了SY及其主要化学成分对2-脱氧核糖降解的抑制作用和对DPPH·的清除能力,同时对红花黄色素B(SYB)抑制2-脱氧核糖降解的作用机制做了初步研究.结果表明SY抑制Fenton反应对2-脱氧核糖的氧化降解的IC50为256.79 μg/mL,对DPPH·清除的IC50值为27.15μg/mL.羟基红花黄色素A(HSYA)和SYB是SY中抗氧化的两个有效成分,抑制2-脱氧核糖的氧化降解的IC50分别为220.68 μg/mL和207.01μg/mL;对DPPH·清除的IC50值分别为55.81 μg/mL和41.25μg/mL.SYB对2-脱氧核糖氧化降解的抑制作用机理研究表明,SYB除了对Fenton反应产生的羟基自由基具有直接清除作用外,又可通过与Fe2+离子的络合作用而阻断Fenton反应产生羟基自由基.由此可知SY具有明显的体外抗氧化活性,SYB和HSYA为其主要抗氧化活性成分.

Study on the antioxidation of safflower yellow

To explore the antioxidant activity of Safflower yellow(SY)and its main active ingredient,the inhibition of 2-deoxyribose degradation induced by hydroxyl radical and the ability of scavenging DPPH·was examined.Hydroxyl radical was produced through the Fenton reaction.The protection ability of SY and its main chemical components on 2-deoxyribose degradation induced by hydroxyl radical,and the ability of scavenging DPPH·were examined in vitro by UV spectrophotometer.At the same time,the mechanism of Safflower yellow B(SYB)inhibiting the degradation of 2-deoxyribose had been studied.Results showed that SY could inhibit the degradation of 2-deoxyribose induced by hydroxyl radical.SY could also eliminate DPPH·effectively.The IC50 was 256.79 μg/mL and 27.15 μg/mL respectively,and both of them showed a dose-effect relationship.Hydroxy safflower yellow A(HSYA)and SYB were two main active compounds in SY.The IC50 of inhibition 2-deoxyribose degradation was 220.68 μg/mL and 207.01 μg/mL,and the IC50 of scavenging DPPH·was 55.81 μg/mL and 41.25 μg/mL respectively.The results of mechanism test showed that,apart from direct scavenging the hydroxyl radicals produced by the Fenton reaction,SYB could also chelated with iron ions and block Fenton reaction.So SY had a significant antioxidation activity,while SYB and HSYA were main ingredients in SY.