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Utilization of microgravity to improve the crystal quality of biologically important proteins: chaperonin-60, GrpE, B-subunit of V-type ATPase, and MIF |
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| Authors: | Ken Kitano Ryo Sasaki Terukazu Nogi Takaaki A Fukami Atsushi Nakagawa Kunio Miki Isao Tanaka |
| Institution: | a Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan b Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan |
| Abstract: | Protein crystallization experiments under microgravity were performed by using commercial vapor diffusion apparatus (CVDA) hardware on the STS-84 shuttle mission. Chaperonin-60, GrpE, the B subunit of V-type ATPase, and the selenomethionine substituted proteins of macrophage migration inhibitory factor (MIF) were selected as target proteins. The results indicate that microgravity positively affected their crystal growth, supporting the practical utilization of microgravity for the protein structure determination. |
| Keywords: | Protein crystallization Microgravity Chaperonin GrpE ATPase Macrophage migration inhibitory factor |
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