Isolation and purification of Pseudostellarin B (cyclic peptide) from <em>Pseudostellaria heterophylla</em> (Miq.) Pax by high-speed counter-current chromatography期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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*Isolation and purification of Pseudostellarin B (cyclic peptide) from Pseudostellaria heterophylla* (Miq.) Pax by high-speed counter-current chromatography**

Authors:	Chao Han Jie Liu Xiaoru Wang

Institution:	^a Department of Chemistry and the Key Laboratory of Analytical Science of the MOE, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China ^b College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, China ^c Qingdao Key Lab of Analytical Technology Development and Standardization of Chinese Medicines, First Institute Oceanography of SOA, Qingdao 266061, China

Abstract:	Pseudostellarin B (cyclic peptide) was isolated and purified from the herbs of Pseudostellaria heterophylla (Miq.) Pax for the first time by high-speed counter-current chromatography (HSCCC) using a two-phase solvent system consisting of n-butanol-ethyl acetate-water (0.6:4.4:5, v/v). The technique can isolate mg levels of the target compound per run with purity better than 96%. The chemical structure of the compound has been positively confirmed by electrospray ionization time of flight (TOF) MS, ¹HNMR, ¹³CNMR and ¹H-¹³CCOSY analyses.

Keywords:	Pseudostellaria heterophylla (Miq ) Pax HSCCC Pseudostellarin B Preparative chromatography
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