Enzymatic reverse FIA method for total phenols determination in urine samples |
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Authors: | Marcos Grünhut Beatriz S Fernández Band |
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Institution: | FIA Laboratory, Department of Chemistry, Universidad Nacional del Sur, Av. Alem 1253, 8000 Bahía Blanca, Buenos Aires, Argentina |
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Abstract: | A reverse flow injection spectrophotometric enzymatic method is proposed to quantify total phenols in urine samples. The polyphenol oxidase (PPO; EC 1.14.18.1) obtained as a crude extract from sweet potato root (Ipomoea batatas) was used as enzymatic catalyze. The detection limit, the sample throughput and relative standard deviation were 7.7 mg l−1 of total phenols, 49 h−1 and 0.9%, respectively. The method was applied to real samples and a recovery study was carried out in order to its validation. |
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Keywords: | Reverse FIA method Polyphenol oxidase Urine samples |
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