| QuEChERS-HPLC法测定土壤中麦角甾醇含量 |
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| 引用本文： | 曹梦,李勇,孙忠祥,兴安,冯晓晓,黄元仿,张红艳. QuEChERS-HPLC法测定土壤中麦角甾醇含量[J]. 分析试验室, 2019, 38(2): 162-166 |
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| 作者姓名： | 曹梦 李勇 孙忠祥 兴安 冯晓晓 黄元仿 张红艳 |
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| 作者单位： | 中国农业大学资源与环境学院,北京100193;农业部华北耕地保育重点实验室,北京100193;国土资源部农用地质量与监控重点实验室,北京100035;中国农业大学理学院,北京,100193 |
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| 摘 要： | 结合QuEChERS法与高效液相色谱(HPLC)技术,建立了一种土壤中麦角甾醇的快速检测方法。以甲醇为提取溶剂,N-丙基-乙二胺(PSA)为吸附剂,实现了样品的快速提取净化;利用Agilent HC-C18色谱柱(250 mm×4. 6 mm,5μm)进行HPLC分离,以甲醇为流动相,紫外检测器于280 nm波长处检测,基质匹配标准溶液外标法定量。结果表明,麦角甾醇在0. 01～10. 00 mg/L浓度范围内具有良好的线性关系(R^2> 0. 999);在添加浓度为0. 5,1. 0和5. 0 mg/kg的添加回收试验中,平均回收率为87. 6%～92. 8%,相对标准偏差(RSD)为0. 82%～3. 0%,方法的定量限为0. 5 mg/kg。该方法可以直接用于土壤样品中麦角甾醇的含量检测,并已成功分析了山西省安太堡露天煤矿的28个土样。
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| 关 键 词： | QUECHERS 高效液相色谱 土壤 麦角甾醇 |
A new QuEChERS-HPLC method for determining ergosterol in soil |
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| CAO Meng,LI Yong,SUN Zhong-xiang,XING An,FENG Xiao-xiao,HUANG Yuan-fang,ZHANG Hong-yan. A new QuEChERS-HPLC method for determining ergosterol in soil[J]. Chinese Journal of Analysis Laboratory, 2019, 38(2): 162-166 |
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| Authors: | CAO Meng LI Yong SUN Zhong-xiang XING An FENG Xiao-xiao HUANG Yuan-fang ZHANG Hong-yan |
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| Affiliation: | (College of Resources and Environmental Sciences,China Agricultural University,Beijing 100193;Key Laboratory of Arable Land Conservation in North China,the Ministry of Agriculture,Beijing 100193;Key Laboratory of Land Quality,Ministry of Land andResources,Beijing 100193;College of Science,China Agricultural University,Beijing 100193) |
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| Abstract: | CAO Meng;LI Yong;SUN Zhong-xiang;XING An;FENG Xiao-xiao;HUANG Yuan-fang;ZHANG Hong-yan(College of Resources and Environmental Sciences,China Agricultural University,Beijing 100193;Key Laboratory of Arable Land Conservation in North China,the Ministry of Agriculture,Beijing 100193;Key Laboratory of Land Quality,Ministry of Land andResources,Beijing 100193;College of Science,China Agricultural University,Beijing 100193) |
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| Keywords: | QuEChERS HPLC Soil Ergosterol |
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