Spectrophotometric determination of advanced products of non-enzymatic glycosylation of lysine by means of their reaction with diazonium salts |
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Authors: | Giovanni Candiano Gian Marco Ghiggeri Gerolamo Delfino Carlo Queirolo Fabrizio Ginevri |
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Institution: | Department of Internal Medicine and Hemodialysis Service, Hospital of Lavagna, Lavagna GE Italy;Gaslini Institute, Genoa Italy |
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Abstract: | A typical product (glycosyl lysine) formed by reaction of lysine with glyceraldehyde is measured at 490 nm after coupling with diazotized sulfanilic acid. Reaction with 4-dimethylaminobenzaldehyde was less satisfactory. |
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