Binding Properties of Water-Soluble Carbosilane Dendrimers |
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Authors: | Elzbieta Pedziwiatr Dzmitry Shcharbin Louis Chonco Paula Ortega F Javier de la Mata Rafael Gómez Barbara Klajnert Maria Bryszewska Ma Angeles Mu?oz-Fernandez |
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Institution: | Department of General Biophysics, University of Lodz, 12/16 Banacha St., 90-237, Lodz, Poland. |
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Abstract: | Dendrimers have been proposed as new carriers for drug delivery. They have distinctive characteristics, such as uniform and
controlled size, monodispersity and modifiable surface group functionality, which make them extremely useful for biomedical
applications. In this study, the binding capacity of water-soluble carbosilane dendrimers was examined. A double fluorimetric
titration method with 1-anilinonaphthalene-8-sulphonic acid (ANS) was used to estimate the binding constant and the number
of binding centers per dendrimer molecule. The data obtained suggest that ANS interacts non-covalently with the dendrimers.
Second generation dendrimers have an open, asymmetric structure that allows them to encapsulate ANS. The ability of the polymers
to interact with DNA was assessed by an ethidium bromide (EB) displacement assay. All the dendrimers studied bound to DNA
in competition with EB, though the strength of binding varied. Dendrimer interactions with a protein (BSA) were tested using
fluorescence quenchers. The dendrimers caused no conformation change in the protein, indicating that interactions between
carbosilane dendrimers and BSA are weak and occur preferentially at the protein surface. |
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Keywords: | Carbosilane dendrimer Drug delivery Fluorimetric titration EB intercalation Fluorescence quenching |
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