氧氟沙星片剂与针剂中有效成分的毛细管区带电泳快速测定

建立了一种简单,快速的毛细管区带电泳法,用于测定氧氟沙星片剂与针剂中有的效成分。背景电解液的组成为１０ｍｍｏ／Ｌ ＫＨ２ＰＯ４与１２ｍｍｏｌ／Ｌ ＣｕＳＯ４的混合溶液,其ＰＨ值为３．６,检测波长为２９０ｎｍ。同时研究了背景电解液中Ｃｕ＾２＋的加入对氧氟沙星的有效电泳淌度以及检测灵敏度的影响。结果表明Ｃｕ＾２＋的加入可以有效地改善氧氟沙星的峰型,提高检测灵敏度。对所建立方法的线性、灵敏度、精密度,回收率等进行了考察,结果表明地适于测定氧氟沙星片剂与针剂中的有效成分的含量。

Determination of Ofloxacin in Ofloxacin Tablet and Intramuscular Injection by Capillary Zone Electrophoresis

A simple and rapid method for the determination of ofloxacin in Ofloxacin tablet and intra- muscular injection is reported by capillary zone electrophoresis. The background buffer solution consists of 10 mmol/L KH2PO4 and 12 mmol/L Cu2 (pH 3.6). The detection wavelength is at 290 nm. The effect of the addition of Cu2 in the buffer on the effective mobility of ofloxacin and the sensitivity was studied. The experimental results showed that the addition of Cu2 could improve the peak shape of ofloxacin and detection sensitivity. The linearity, sensitivity, precision and recovery of the method were also investi- gated. The results were satisfactory.