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Analysis of oxidized multi-walled carbon nanotubes in single K562 cells by capillary electrophoresis with laser-induced fluorescence |
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| Authors: | Hua Xiao Lisong Yang Hanfa Zou Ling Yang X. Chris Le |
| Affiliation: | (1) National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, People’s Republic of China;(2) Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, People’s Republic of China;(3) Department of Public Health Sciences, Faculty of Medicine, University of Alberta, 10-102 CSB, Edmonton, Alberta, T6G 2G3, Canada |
| Abstract: | Short oxidized multi-walled carbon nanotubes (CNT) were derivatized with fluorescein isothiocyanate (FITC). Capillary electrophoresis coupled with laser-induced fluorescence (CE–LIF) was then used to separate and detect the fluorescently labeled carbon-nanotube probes (CNTP) in multidrug-resistant cells (K562A) and the parent cells (K562S). Greater expression of P-glycoprotein in K562A cells than in K562S cells was confirmed by use of anti-P-glycoprotein antibody and flow-cytometric analysis. Analyses of CNTP in both cell lines using both CE–LIF and flow cytometry showed that CNTP could traverse the cellular membrane without being pumped out by P-glycoprotein. The CNTP distributed in both cell lines was analyzed at the single cell level and the results were compared with those from analysis of ten cells and of the lysate from bulk cells. The results revealed the CE–LIF method could be used for quantitative analysis of CNT in single cells in studies of drug delivery and multidrug resistance.  |
| Keywords: | Multi-walled carbon nanotubes Single-cell analysis Multidrug resistance Capillary electrophoresis Laser-induced fluorescence |
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