On-line identification of sugarcane (Saccharum officinarum L.) methoxyflavones by liquid chromatography-UV detection using post-column derivatization and liquid chromatography-mass spectrometry |
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| Authors: | Colombo Renata a Yariwake Janete H Queiroz Emerson F Ndjoko Karine Hostettmann Kurt |
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| Institution: | 1. Instituto de Química de São Carlos, Universidade de São Paulo, Caixa Postal 780, 13560-970 São Carlos, SP, Brazil;2. Laboratoire de Pharmacognosie et Phytochimie, École de Pharmacie Genève-Lausanne, Université de Genevè, Quai Ernest Ansermet 30, 1211 Genève 4, Genève, Switzerland |
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| Abstract: | Sugarcane (Saccharum officinarum L., Gramineae) bagasse and leaves were investigated for their flavonoid content and transgenic sugarcane ("Bowman-Birk" and "Kunitz") was compared with non-modified ("control") plants. Analyses were carried out by high-performance liquid chromatography coupled to diode array UV detection (LC/UV), also using post-column addition of shift reagents, and tandem MS (atmospheric pressure chemical ionization-MS/MS and collision-induced dissociation-MS). On-line UV and MS data demonstrated the presence of methoxyflavone glycosides and aglycones in a total of seven compounds. Three naturally occurring flavones glycosides and two unusual erythro- and threo-diastereoisomeric flavolignan 7-O-glucosides were identified together with their aglycones. |
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| Keywords: | LC/UV LC/MS Flavonoids Transgenic plants |
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