A CE Method for Measuring Phototoxicity in Vitro |
| |
| Authors: | I. Hinneburg S. Kempe H.-H. Rüttinger R.H.H. Neubert |
| |
| Affiliation: | 1. Institute of Pharmaceutics and Biopharmaceutics, Department of Pharmacy, Martin-Luther-University Halle-Wittenberg, Wolfgang-Langenbeck-Stra?e 4, 06120, Halle, Germany
2. Institute of Pharmaceutical Chemistry, Department of Pharmacy, Martin-Luther-University Halle-Wittenberg, 06120, Halle, Germany
|
| |
| Abstract: | This paper presents an experimental setup which employs capillary electrophoresis with electrochemical and UV detection to test phototoxicity of plant extracts and components in terms of oxygen consumption and generation of reactive oxygen species upon irradiation with visible light. The experimental setup was used to test the phototoxicity of different buckwheat extracts and individual plant derived substances. The buckwheat extracts showed differences in their phototoxic behavior which might be due to different phytochemical composition. Screening of individual components revealed that rutin and quercetin alone were not phototoxic, but quercetin in combination with hypericin and chlorophyll caused considerable oxygen consumption. It has been demonstrated that the apparatus is a valuable tool to screen in vitro potential phototoxic reactions of plant extracts and individual constituents. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
