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Headspace Liquid-Phase Microextraction of Short-Chain Fatty Acids in Plasma, and Gas Chromatography with Flame Ionization Detection |
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| Authors: | L Tan X P Zhao X Q Liu H X Ju J S Li |
| Institution: | 1. Key Laboratory of Analytical Chemistry for Life Science (MOE), Department of Chemistry, Nanjing University, Nanjing, 210093, China 2. Research Institute of General Surgery, Jinling Hospital, Nanjing University, Nanjing, 210002, China 3. Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, 210009, China |
| Abstract: | A novel method for analysis of short-chain fatty acids (SCFA) in blood plasma has been developed by combining headspace liquid-phase microextraction (HS-LPME) and gas chromatography with flame ionization detection. HS-LPME conditions, for example working solution volume, salt concentration, and extraction temperature and time, were optimized by means of an L9 (34) orthogonal array design. The effects on extraction efficiency of the type of solvent and the volume of the microdrop, the pH of the sample matrix, and the stirring rate were examined. Under optimum conditions the linear calibration ranges for SCFA were from 0.08 to 80 µg mL?1 (r 2>0.9921) with detection limits of 0.02 to 0.08 µg mL ?1 and relative standard deviation less than 9.81%. Recovery from spiked blood plasma samples was more than 70.5%. The method was satisfactorily applied to analysis of SCFA in rat blood plasma samples from the blind-loop model. |
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