Strategies for Analyzing Cephalosporins by Microemulsion Electrokinetic Chromatography |
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Authors: | P Puig F Borrull M Calull C Aguilar |
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Institution: | 1. Department of Analytical Chemistry and Organic Chemistry, University Rovira i Virgili, Marcel·lí Domingo, 43007, Tarragona, Spain
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Abstract: | We studied microemulsion electrokinetic chromatography (MEEKC) for the analysis of cefazolin, cefoperazone and cephalexin. We compared the separation of the cephalosporins obtained by MEEKC at a high pH and at a low pH. Good separations of the compounds were achieved within 9 min by both methods. To reduce the analysis time, we used the "short-end" injection technique. We also present two on-line preconcentrations of the analytes by sample stacking, reversed electrode polarity stacking mode (REPSM) (at high-pH) and stacking with reverse migrating pseudostationary phase (SRMP) (at low-pH). We also report parameters for validation such as linearity and limit of detection. Our results show that the developed methods are suitable for analyzing these three cephalosporins. |
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