Parallel Raman microspectroscopy using programmable multipoint illumination |
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Authors: | Qi Ji Shih Wei-Chuan |
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Institution: | Department of Electrical & Computer Engineering, University of Houston 4800 Calhoun Rd., Rm N308 Eng. Bldg 1, Houston, Texas 77204, USA. wshih@uh.edu |
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Abstract: | We present a novel parallel Raman microspectroscopy scheme for simultaneously collecting Raman spectra from multiple points. This scheme is realized by projectinga multiple-point laser illumination pattern using a spatial light modulator (SLM) and wide-field Raman imaging collection. We demonstrate the performance of this scheme using uniform samples, trapped polymer microparticles and fixed polymer microparticles with mixed molecular composition within a 50×50 μm(2) field of view. This scheme enables the acquisition of Raman spectra from as many as 40 points simultaneously using a single illumination pattern and detector recording frame without scanning. |
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