| Large scale synthesis of oligoribonucleotides on PEG by the phosphite triester approach |
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| 引用本文： | XU,Zu-Sheng YANG,Zai-Wan WANG,YuShanghai Institute of Organic Chemistry,Chinese Academy of Sciences,354 Fenglin Lu,Shanghai 200032,China. Large scale synthesis of oligoribonucleotides on PEG by the phosphite triester approach[J]. 中国化学, 1995, 13(3): 246-250. DOI: 10.1002/cjoc.19950130309 |
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| 作者姓名： | XU Zu-Sheng YANG Zai-Wan WANG YuShanghai Institute of Organic Chemistry Chinese Academy of Sciences 354 Fenglin Lu Shanghai 200032 China |
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| 作者单位： | XU，Zu-Sheng YANG，Zai-Wan WANG，YuShanghai Institute of Organic Chemistry，Chinese Academy of Sciences，354 Fenglin Lu，Shanghai 200032，China |
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| 摘 要： | Large scale synthesis of oligoribonucleotides has been successfully performed on PEG support by the phosphoramidite approach using t-butyldimethylsilyl to protect the 2'-hydroxyl group of ribonucleoside. By means of this procedure, the dodecamer r(AGUGGUCUUUGU) was synthesized in 98.1% average coupling yield, and 55 mg pure product was obtained from one gram of functionalized PEG.
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Large scale synthesis of oligoribonucleotides on PEG by the phosphite triester approach |
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| XU,Zu-Sheng YANG,Zai-Wan WANG,Yu. Large scale synthesis of oligoribonucleotides on PEG by the phosphite triester approach[J]. Chinese Journal of Chemistry, 1995, 13(3): 246-250. DOI: 10.1002/cjoc.19950130309 |
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| Authors: | XU Zu-Sheng YANG Zai-Wan WANG Yu |
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| Affiliation: | XU,Zu-Sheng YANG,Zai-Wan WANG,YuShanghai Institute of Organic Chemistry,Chinese Academy of Sciences,354 Fenglin Lu,Shanghai 200032,China |
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| Abstract: | Large scale synthesis of oligoribonucleotides has been successfully performed on PEG support by the phosphoramidite approach using t-butyldimethylsilyl to protect the 2'-hydroxyl group of ribonucleoside. By means of this procedure, the dodecamer r(AGUGGUCUUUGU) was synthesized in 98.1% average coupling yield, and 55 mg pure product was obtained from one gram of functionalized PEG. |
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| Keywords: | Oligoribonucleotides large scale synthesis phosphoramidite approach PEG support. |
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