垃圾渗滤液处理厂活性污泥微生物种群结构和功能分析

对垃圾渗滤液处理厂活性污泥的16S rRNA基因和宏基因组进行高通量测序并分析,探究垃圾渗滤液处理厂活性污泥微生物的种群结构、氮循环和有机污染物降解相关功能微生物和功能基因.结果表明,Cal-ditrichaeota门微生物丰度最高(58.77％),其次是Proteobacteria(16.80％)和Bacteroid...

Microbial Structure and Function of Activated Sludge in Landfill Leachate Treatment Plant

16S rRNA gene and metagenomic sequencing was applied to study the structure of microbial community, functional microorganisms and genes in activated sludge of landfill leachate treatment. The results showed that Calditrichaeota was the most abundant phylum (58.77%), followed by Proteobacteria (16.80%) and Bacteroides (6.19%). Calrithrix was the dominant genus of the activated sludge (58.77%). Five nitrogen cycle pathways were detected to form the microbial community of activated sludge, including nitrification, denitrification, assimilation nitrate reduction, dissimilation nitrate reduction and nitrogen fixation. Denitrification genes were the most abundant among nitrogen cycling process (78.84%), which mainly distributed in Calditrichaeota, Proteobacteria and Choroflexi. Nitrification was achieved by ammonia oxidizing bacteria Nitrosomonas. There were abundant organic pollutants degradation genes in activated sludge. Proteobacteria, Bacteroides, Planctomycetes, Calditrichaeota and Choroflexi were typical functional microorganisms that can degrade organic pollutants.