基质固相分散-反相液相色谱法测定蔬菜中二甲戊乐灵农药残留

应用基质固相分散-反相液相色谱技术建立了提取、检测蔬菜中二甲戊乐灵农药残留的分析方法。研究了提取及测定条件对检测二甲戊乐灵的影响,确定了最佳提取条件:萃取吸附剂为弗罗里硅土,样品与弗罗里硅土吸附剂的比例为1∶4,洗脱剂为20 mL乙酸乙酯。二甲戊乐灵的测定线性范围为0.02~2.0 mg/L、r0.999。应用此方法测定了某些蔬菜样品中二甲戊乐灵农药残留,测定相对标准偏差为2.2%~5.2%。在几种蔬菜中的加标回收率为85%~93%,当样品为0.5 g时,方法检出限在5.5~10 ng范围内。

Determination of pendimethalin pesticide residue in vegetables by matrix solid phase dispersion-reversed phase liquid chromatography

An analytical method for the extraction and detection of pendimethalin pesticide residues in vegetables by using matrix solid phase dispersion-reversed phase liquid chromatography (MSPD-RPLC) was established. The effects of extraction and determination conditions on the detection of pendimethalin were studied;and the optimum extraction conditions were as follows: the extraction sorbent, the ratio of sample to florisil sorbent and the eluting solvent were determination of pendimethalin was 0.02～2.0 mg/L with r>0.999. The pendimethalin pesticide residues in some vegetable samples were determined by application of the proposed method, and the RSDs of determination were in the range of 2.2%～5.2%. The recoveries of standard addition in some of spiked vegetable samples varied from 85% to 93%, and the detection limits of the method ranged from 5.5 to 10 ng when sample was 0.5 g.