A new bio-mimetic sensor, CuO/PANA@PSDS/GCE, was prepared, it exhibited a better electrocatalytic activity toward the reduction of the H2O2 compared with that of the CuO/GCE, PANA@PSDS/GCE, and GCE. Its increased catalytic response was due to the polyaniline doped (PANA@PSDS) film, which enlarges the specific surface area of the electrode, and increases the loading of the CuO nano-particles.