Characterization of selenium species in biological extracts by enhanced ion-pair liquid chromatography with inductively coupled plasma-mass spectrometry and by referenced electrospray ionization-mass spectrometry |
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| Affiliation: | 1. Department of Chemistry, Lederle Graduate Research Tower A, University of Massachusetts, Box 35410, Amherst, MA 01003-4510, USA;2. Department of Chemistry, SUNY-Albany, Albany, NY 12222, USA;1. School of Life Sciences, Shandong University of Technology, Zibo, Shandong, 255000, China;2. School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, 255000, China;1. Department of Physics, Bangalore University, Jnanabharathi Campus, Bengaluru, 560 056, India;2. New Chemistry Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur P. O, Bangalore, 560064, India;3. UGC-DAE-Consortium for Scientific Research, Mumbai Centre, BARC Campus, Mumbai – 400085, India;1. Laboratory of Analytical Chemistry for Research and Development (QUIANID), Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo, Padre J. Contreras 1300, 5500 Mendoza, Argentina;2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina;2. Key Laboratory for Agro-Food Risk Assessment of Ministry of Agriculture, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China;2. Agriculture and Agri-Food Canada, Québec Research and Development Centre, Québec, Canada GIV 2J3;3. Université de Montréal, Département de Biomédecine Vétérinaire, Québec, Canada J2S 2M2 |
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| Abstract: | Selenium is an essential nutrient for humans; selenium compounds catalyze intermediate metabolism reactions and inhibit the toxic effects of heavy metals such as arsenic, cadmium and mercury. Some extracts of selenium-enriched biological materials show cancer preventive effects, tentatively attributable to the biological functions of selenoamino acids. An improved ion pair chromatographic method with methodological enhancements for the separation, qualitative and quantitative determination of non-volatile selenium compounds extracted from different samples has been developed using ICP-MS as an element-selective detector. Separation power early in the chromatogram was increased to baseline separation in the standard mixture as a result of decreasing spray chamber size from 97 to 14 ml, and increasing trifluoracetic acid (TFA) concentration in the mobile phase from 0.1 to 0.6%. The former pH was restored by the addition of ammonia to the mobile phase, which also served to increase the column recovery of inorganic anions. Calibration curves for different selenoamino acids showed statistically different behavior. Biological sample extracts were characterized using HPLC-ICP-MS. Mass spectral behavior of selenoamino acids, using electrospray and ion trap technology with direct infusion and liquid chromatographic sample introduction, is also reported. |
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