An alternative paper based tissue washing method for mass spectrometry imaging: localized washing and fragile tissue analysis |
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| Authors: | van Hove Erika R Amstalden Smith Donald F Fornai Lara Glunde Kristine Heeren Ron M A |
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| Institution: | (1) FOM Institute AMOLF, Science Park 104, 1098 XG Amsterdam, The Netherlands;(2) The Netherlands Proteomics Centre, Utrecht University, H.R. Kruytgebouw, Utrecht, The Netherlands;(3) JHU In Vivo Cellular and Molecular Imaging Center, The Russell H. Morgan Department of Radiology and Radiological Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA; |
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| Abstract: | Surface treatment of biological tissue sections improves detection of peptides and proteins for mass spectrometry imaging.
However, liquid surface treatments can result in diffusion of surface analytes and fragile tissue sections can be easily damaged
by typical washing solvents. Here, we present a new surface washing procedure for mass spectrometry imaging. This procedure
uses solvent wetted fiber-free paper to enable local washing of tissue sections for mass spectrometry imaging and tissue profiling
experiments. In addition, the method allows fragile tissues that cannot be treated by conventional washing techniques to be
analyzed by mass spectrometry imaging. |
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