HPLC法测定百合固金颗粒中芍药苷和甘草苷

建立同时测定百合固金颗粒中芍药苷和甘草苷含量的高效液相色谱法。采用Welch Ultimate XB–C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈–0.1%磷酸(体积比为14∶86),流速为1.0 m L/min,柱温为30℃,检测波长为230 nm。百合固金颗粒中芍药苷在14.91~149.12 ng,甘草苷在34.75~347.52 ng范围内均有良好线性,线性相关系数分别为0.999 5,0.999 2,平均回收率分别为96.7%,95.9%,测定结果的相对标准偏差小于3%(n=6)。该方法操作简单,专属性强,准确度高,重复性好,可以有效地控制百合固金颗粒的质量。

Determination of Paeoniflorin and Liquoritin in Baiheguji Granules by HPLC

A method was established for the simultaneous determination of paeoniflorin and liquoritin content in Baiheguji granules by HPLC.Welch UltimateXB-C18 colum (250 mm× 4.6 mm,5 μ m) was adopted with acetonitrile0.1％ phosphoric acid (14:86) as the mobile phase at flow rate of 1.0 mL/min.Detection wave length was 230 nm,colum temperature was 30 ℃.The content of Paeoniflorin and Liquoritin was linear with the peak area in the range of 14.91-149.12 ng and 34.75-347.52 ng,respectively,and the correlation coefficient was 0.999 5,0.999 2,respectively.The average recovery were 96.7％,95.9％,respectivly,and the relative standard deviation of detection results was less than with 3％(n=6).The method has advantages of simple operation,high accuracy stability and good reproducibility.It can be used for quality control of Baiheguji granules effectively.