| Sensitivity—enhanced Experiments for the Measurement of J and Dipolar Coupling Constants |
| |
| 引用本文： | LIN，Dong－Hai LIAO，Xin－Li.Sensitivity—enhanced Experiments for the Measurement of J and Dipolar Coupling Constants[J].中国化学,2002,20(7):623-628. |
| |
| 作者姓名： | LIN，Dong－Hai LIAO，Xin－Li |
| |
| 作者单位： | [1]DepartmentofBiochemistry,TheHongKongUniversityofScience＆Technology,HongKong,China [2]DepartmentofChemistry,XiamenUniversity,Fujian361005,China |
| |
| 基金项目： | ProjectsupportedbytheNationalNaturalScienceFoundationofChina (No.19975 0 38) |
| |
| 摘 要： | A sensitivity-enhanced IPAP NMR experiment was described in this paper,which separates the ^1H-^15N doublets into two different spectra to alleviate the problem of resonance overlaps and achieve the accurate measurement of J and residual dipolar coupling constants in proteins.This experiment offered 20%-60% sensitivity enhancement over the original IPAP experiment,and therefore produced more measurable resonances.Pulsed field gradient was used for coherence selection.Water-flip-back approach was used for water suppression.The sensitivity-enhanced IPAP experiment was employed in the measurement of ^1JNH and ^1DNH constants of the protein UBC9.
|
| 关 键 词： | 丁耦合常数 灵敏性提高试验 两极耦合常数 蛋白质 共振叠加 |
Sensitivity-enhanced Experiments for the Measurement of J and Dipolar Coupling Constants |
| |
| LIN,Dong Hai LIAO,Xin Li a.Sensitivity-enhanced Experiments for the Measurement of J and Dipolar Coupling Constants[J].Chinese Journal of Chemistry,2002,20(7):623-628. |
| |
| Authors: | LIN Dong Hai LIAO Xin Li a |
| |
| Institution: | LIN,Dong Hai LIAO,Xin Li a Department of Biochemistry,The Hong Kong University of Science & Technology,Hong Kong,China b Department of Chemistry,Xiamen University,Fujian 361005,China |
| |
| Abstract: | A sensitivity enhanced IPAP NMR experiment was described in this paper, which separates the 1 H 15 N doublets into two different spectra to alleviate the problem of resonance overlaps and achieve the accurate measurement of J and residual dipolar coupling constants in proteins. This experiment offered 20%-60% sensitivity enhancement over the original IPAP experiment, and therefore produced more measurable resonances. Pulsed field gradient was used for coherence selection. Water flip back approach was used for water suppression. The sensitivity enhanced IPAP experiment was employed in the measurement of 1 J NH and 1 D NH constants of the protein UBC9. |
| |
| Keywords: | J coupling residual dipolar coupling liquid crytalline media spin state separated spectra sensitivity enhancement |
| 本文献已被 CNKI 维普 等数据库收录！ |
