Isolation,purification, and enzymatic activity of cellulase components of the fungus Aspergillus terreus |
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| Authors: | Sh. Ya. Mirzaakhmedov Zh. F. Ziyavitdinov Z. R. Akhmedova A. B. Saliev D. T. Ruzmetova Kh. B. Ashurov Dimitrios Fessas Stefania Iametti |
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| Affiliation: | (1) Institute of Bioorganic Chemistry, 100125 Tashkent, pr. Kh. Abdullaeva, 83;(2) Institute of Microbiology, 100128 Tashkent, ul. A. Kadyri, 7b;(3) Institute of Electronics, 100125 Tashkent, ul. F. Khodzhaeva, 33;(4) University of Milan, DISTAM, via Celoria 2, 20133 Milano, Italy |
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| Abstract: | The accumulation dynamics of cellulolytic enzymes in culture media of the basidiomycete fungi Panus tigrinus, Pleurotus ostreatus, Fomes fomentarus, and the micromycete Aspergillus terreus were studied during a long incubation period. It was found that A. terreus was the most active producer of cellulolytic enzymes among the studied fungi. Two protein fractions with cellulase activity were isolated using gel filtration and ion-exchange chromatography. PAAG electrophoresis showed that fraction-I consisted of four components; fraction-II, an electrophoretically homogeneous protein. __________ Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 489–491, September–October, 2007. |
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| Keywords: | cellulase endoglucanase activity chromatography Aspergillus terreus electrophoresis |
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