A comparison of NCO and NCA transfer methods for biological solid-state NMR spectroscopy |
| |
Authors: | Loening Nikolaus M Bjerring Morten Nielsen Niels Chr Oschkinat Hartmut |
| |
Institution: | Department of Chemistry, Lewis & Clark College, 0615 SW Palatine Hill Road, Portland, OR 97219, USA. loening@lclark.edu |
| |
Abstract: | Three different techniques (adiabatic passage Hartman-Hahn cross-polarization, optimal control designed pulses, and EXPORT) are compared for transferring (15)N magnetization to (13)C in solid-state NMR experiments under magic-angle-spinning conditions. We demonstrate that, in comparison to adiabatic passage Hartman-Hahn cross-polarization, optimal control transfer pulses achieve similar or better transfer efficiencies for uniformly-(13)C,(15)N labeled samples and are generally superior for samples with non-uniform labeling schemes (such as 1,3- and 2-(13)C glycerol labeling). In addition, the optimal control pulses typically use substantially lower average RF field strengths and are more robust with respect to experimental variation and RF inhomogeneity. Consequently, they are better suited for demanding samples. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|