Design of a multinuclear Zn(II) complex as a new molecular probe for fluorescence imaging of His-tag fused proteins |
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Authors: | Fujishima Sho-hei Nonaka Hiroshi Uchinomiya Sho-hei Kawase Yoshiyuki Alex Ojida Akio Hamachi Itaru |
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Affiliation: | Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto, 615-8510, Japan. |
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Abstract: | A Zn(II) complex (Zn(II)-Ida) was designed as the new fluorescent probe for His-tag fused proteins. Thanks to the tight binding ability to histidine-rich sequences and bright fluorescence property of the Cy5-appended Zn(II)-Ida probes, selective and clear fluorescent imaging of the His-tag fused G-protein coupled receptors on live cell surfaces was carried out. |
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