Use of liquid chromatography-tandem mass spectrometry for the analysis of pentoxifylline and lisofylline in plasma |
| |
Authors: | Kyle Patrick B Adcock Kim G Kramer Robert E Baker Rodney C |
| |
Institution: | Department of Pharmacology and Toxicology University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216, USA. pkyle@pharmacology.umsmed.edu |
| |
Abstract: | A method was developed for the quantitation of pentoxifylline 1-(5-oxohexyl)-3,7-dimethylxanthine] and a primary active metabolite, lisofylline 1-(5-hydroxyhexyl)-3,7-dimethylxanthine], using high-performance liquid chromatography (HPLC)-tandem mass spectrometry. This method was developed in order to overcome problems encountered with HPLC-ultraviolet detection. The operating parameters of the electrospray interface (PE SCIEX, TurboIon Spray) and lens voltages of the triple-quadrupole detector (PE SCIEX 365) were optimized in positive ion mode to obtain the best sensitivity of the analytes. Collision-induced dissociation was used to produce fragment ions, and multiple reaction monitoring was used to quantitate pentoxifylline (m/z 279/181) and lisofylline (m/z 263/181). Dichloromethane was used to extract the drug, metabolite, and the internal standard (3-isobutyl-1-methylxanthine) from plasma. A reverse-phase C8(2) 150 x 1.0 mm HPLC column was used to resolve all three compounds in less than 6 min. Calibration curves were generated using peak area and were linear from 1 to 1000 ng/mL (R(2) > 0.99). The small sample volume, ease of extraction, and sensitivity provide advantages over more conventional methods of quantitation. |
| |
Keywords: | pentoxifylline LC–MS electrospray ionization |
本文献已被 PubMed 等数据库收录! |
|