Development of a flow injection method for monitoring cell membrane damage of wine lactic acid bacteria |
| |
Authors: | André F Torres Paulo A R Mesquita Francisco M Campos José A Couto Ildikó V Tóth António O S S Rangel Timothy A Hogg |
| |
Institution: | (1) Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, P-4200-072 Porto, Portugal |
| |
Abstract: | A flow injection analysis (FIA) system was developed for the determination of phosphate efflux from wine lactic acid bacteria
(Oenococus oeni and Lactobacillus hilgardii) as an indication of cell membrane damage. The system allowed the direct injection of the cell suspension, avoiding the filtration
step, with minimum sample treatment and minimized reagent consumption. The developed system is characterized by a linear concentration
zone between 3.23 × 10−5 and 4.84 × 10−4 mol L−1 PO4
3− and repeatability better than 2.9%. Bacterial suspensions were exposed to a chemical stress with phenolic acids and injected
in the FIA system at regular intervals. The extracellular concentration of phosphate was measured spectrophotometrically.
The experimental results obtained indicate that hydroxycinnamic acids (p-coumaric, ferulic and caffeic) induced faster phosphate leakage rates than hydroxybenzoic acids (vanillic and p-hydroxybenzoic) in both strains tested, which could be related to their higher lipophilic character. |
| |
Keywords: | : Flow injection analysis membrane damage phenolic acids phosphate efflux wine lactic acid bacteria |
本文献已被 SpringerLink 等数据库收录! |