1. College of Biological, Chemical Science and Engineering, Jiaxing University, Jiaxing, 314001, China
Abstract:
Novel imidazole fluorescent ionic liquids with anthracene groups (ImS-FILA) were synthesized for the first time to act as fluorescent probes. They were developed for the determination of superoxide anion radicals (O2?-) in an aqueous system. O2?- was produced by pyrogallol autoxidation. The fluorescence of ImS-FILA was quenched by superoxide anion radicals. The π-bond structure of the fluorescent molecules was oxidized and damaged. This method is very simple and sensitive. The linear range of sensitivity was 1–70 μM ImS-FILA, and the detection limit for reactive oxygen species was 0.1 μM. This method was used to detect superoxide radicals in papaya and garlic, with satisfactory results. Further work is needed to demonstrate the utility of this method in detecting reactive oxygen species in a biological aqueous system.
Figure
Reaction of fluorescent probes with O2?- and the fluorescence change