Study on the interaction between a fluorine-containing amphiphilic cationic copolymer and nucleic acid by resonance light scattering technique |
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Authors: | Ling Li Gong Wu Song |
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Institution: | Ministry of Education, Key Laboratory for the Synthesis and Application of Organic Function Molecules, Hubei University, Wuhan City, Hubei Province 430062, People's Republic of China |
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Abstract: | A novel fluorine-containing amphiphilic cationic copolymer P(HFMA-St-MOTAC)-g-PEG was synthesized as a new probe to detect DNA based on the RLS technique. The aggregation of P(HFMA-St-MOTAC)-g-PEG on the molecular surface of DNA occurred under pH 4.0-7.0 resulted in an enhanced resonance light-scattering (RLS) peaks at 370 nm, 400 nm, 420 nm and 470 nm. The intensity of resonance light-scattering was found to be proportional to the concentration of DNA. The detection limit was 5.8 μg L−1. It was found that the P(HFMA-St-MOTAC)-g-PEG has strong interaction with DNA as confirmed by the resonance light scattering (RLS) spectroscopy, UV spectra, IR spectra, and TEM. |
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Keywords: | Fluorinated cationic amphiphilic graft copolymer DNA Interaction |
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