Quadruplex DNA‐Stabilising Dinuclear Platinum(II) Terpyridine Complexes with Flexible Linkers |
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| Authors: | Dale L Ang Dr Benjamin W J Harper Dr Leticia Cubo Dr Oscar Mendoza Prof Ramon Vilar Prof Janice Aldrich‐Wright |
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| Institution: | 1. Nanoscale Organisation and Dynamics Group, School of Science and Health, Western Sydney University, Penrith, Australia;2. Department of Chemistry, Imperial College London, London, UK;3. Institute of Chemical Biology, Imperial College London, London, UK |
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| Abstract: | Four dinuclear terpyridineplatinum(II) (Pt–terpy) complexes were investigated for interactions with G‐quadruplex DNA (QDNA) and duplex DNA (dsDNA) by synchrotron radiation circular dichroism (SRCD), fluorescent intercalator displacement (FID) assays and fluorescence resonance energy transfer (FRET) melting studies. Additionally, computational docking studies were undertaken to provide insight into potential binding modes for these complexes. The complexes demonstrated the ability to increase the melting temperature of various QDNA motifs by up to 17 °C and maintain this in up to a 600‐fold excess of dsDNA. This study demonstrates that dinuclear Pt–terpy complexes stabilise QDNA and have a high degree of selectivity for QDNA over dsDNA. |
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| Keywords: | circular dichroism DNA FRET G-quadruplexes platinum |
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