壳聚糖固定化亚铁Schiff碱配合物的研究

利用具有多种生物医用活性的高分子壳聚糖的轴向配位作用合成了壳聚糖-N-亚水杨基氨基酸Schiff碱亚铁配合物,采用IR光谱、元素分析、荧光光谱、紫外-可见吸收光谱和热分析等分析手段对配体及高分子配合物进行了表征,推测了其结构.以核黄素光化学氧化-羟胺-偶氮比色法研究了该化合物对超氧离子自由基的清除能力.结果表明,壳聚糖固定化的N-亚水杨基氨基酸Schiff碱亚铁配合物对超氧离子自由基的清除能力有所降低,但仍具有较强的抑制作用.

Synthesis and Characterization of the Complex of Chitosan-immobilization Ferrous-N-salicylidene Glycine Schiff Base

Chitosan′s ramification derived from chitosan and the schiff base and their ferrous complex have been synthesized and characterized by elementary analysis, IRand UV-Vis spectra, fluorescence spectrophotometry and thermal analysis.The Schiff base was derived from salicyl aldehyde and glycine.It was found that the coordination is realized through binding of Nin chitosan with the Fe(Ⅱ) in ferrous N-salicylidene glycine complex.The effect of this complex on the elimination of superoxide anion waSInvestigated by the decrease in the absorbance at 550nm in the system of vitamin B₂-hydroxylamine-anilinesulfonic acid-N-(1-naphthyl)ethylenediamine.Comparing the effect of ferrous N-salicylidene glycine complex on the elimination of superoxide anion with chitosan-ferrous-Schiff base complex′, it was found that the eliminative effect of the chitosan-ferrous-schiff base complex decreased in a sort, but still remained the stronger ability of eliminating superoxide anion.