| 分子光谱法研究铝酞菁与牛血红蛋白的相互作用 |
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| 引用本文： | 张红梅,王彦卿,张根成,吴琳,陶为华,费正皓,刘总堂. 分子光谱法研究铝酞菁与牛血红蛋白的相互作用[J]. 分析试验室, 2007, 26(3): 118-122 |
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| 作者姓名： | 张红梅 王彦卿 张根成 吴琳 陶为华 费正皓 刘总堂 |
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| 作者单位： | 江苏省滩涂生物资源与环境保护重点建设实验室,盐城师范学院应用化学与环境工程研究所,盐城,224002;江苏省滩涂生物资源与环境保护重点建设实验室,盐城师范学院应用化学与环境工程研究所,盐城,224002;江苏省滩涂生物资源与环境保护重点建设实验室,盐城师范学院应用化学与环境工程研究所,盐城,224002;江苏省滩涂生物资源与环境保护重点建设实验室,盐城师范学院应用化学与环境工程研究所,盐城,224002;江苏省滩涂生物资源与环境保护重点建设实验室,盐城师范学院应用化学与环境工程研究所,盐城,224002;江苏省滩涂生物资源与环境保护重点建设实验室,盐城师范学院应用化学与环境工程研究所,盐城,224002;江苏省滩涂生物资源与环境保护重点建设实验室,盐城师范学院应用化学与环境工程研究所,盐城,224002 |
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| 基金项目： | 江苏省滩涂生物资源与环境保护重点建设实验室基金 |
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| 摘 要： | 利用紫外可见吸收光谱和荧光光谱研究了在生理pH条件下铝酞菁与牛血红蛋白(BHb)的相互作用.实验结果表明:铝酞菁分子与BHb发生反应生成基态复合物,导致BHb内源荧光的猝灭,该猝灭属于静态猝灭.测定了不同温度下该反应的表观结合常数、结合位点数及结合热力学参数,热力学参数的变化表明铝酞菁与BHb之间以静电和疏水作用力为主;根据Frster能量转移理论,测得供体与受体间结合距离r和能量转移效率E;并用同步荧光光谱法探讨了铝酞菁对BHb构象的影响.
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| 关 键 词： | 铝酞菁 牛血红蛋白 紫外光谱 荧光光谱 热力学参数 |
| 文章编号： | 1000-0720（2（107）03-118-05 |
| 修稿时间： | 2006-05-232006-08-10 |
Spectroscopic studies of interaction between aluminium phthalocyanine and bovine hemoglobin (BHb) |
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| ZHANG Hong-mei,WANG Yan-qing,ZHANG Gen-cheng,WU Lin,TAO Wei-hua,FEI Zheng-hao,LIU Zong-tang. Spectroscopic studies of interaction between aluminium phthalocyanine and bovine hemoglobin (BHb)[J]. Chinese Journal of Analysis Laboratory, 2007, 26(3): 118-122 |
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| Authors: | ZHANG Hong-mei WANG Yan-qing ZHANG Gen-cheng WU Lin TAO Wei-hua FEI Zheng-hao LIU Zong-tang |
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| Affiliation: | Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers College, Yancheng 224002 |
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| Abstract: | The interaction between aluminium phthalocyanine(AlPc) and bovine hemoglobin(BHb) was investigated using UV/Vis and fluorescence.The experimental results showed that the fluorescence quenching of BHb by AlPc is a result of the formation of AlPc-BHb complex;static quenching was confirmed to result in the fluorescence quenching.The binding site number n,apparent binding constant KA and corresponding thermodynamic parameters were measured at different temperatures.The electrostatic and hydrophobic interactions play a major role in stabilizing the complex.The binding distance and energy-transfer efficiency E between AlPc and BSA were obtained according to fluorescence resonance energy transfer;the effect of AlPc on the conformation of BHb was analyzed using synchronous fluorescence spectroscopy. |
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| Keywords: | Aluminium phthalocyanine BHb UV/Vis spectroscopy Fluorescence spectroscopy Thermodynamic parameters |
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