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H2‐Fueled ATP Synthesis on an Electrode: Mimicking Cellular Respiration |
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| Authors: | Dr Óscar Gutiérrez‐Sanz Dr Paolo Natale Dr Ileana Márquez Dr Marta C Marques Sonia Zacarias Dr Marcos Pita Dr Inês A C Pereira Dr Iván López‐Montero Dr Antonio L De Lacey Dr Marisela Vélez |
| Institution: | 1. Instituto de Catálisis y Petroleoquímica, CSIC, Madrid, Spain;2. Universidad Complutense de Madrid, Madrid, Spain;3. Instituto de Investigación i+12 hospital 12 de Octubre, Madrid, Spain;4. Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal |
| Abstract: | ATP, the molecule used by living organisms to supply energy to many different metabolic processes, is synthesized mostly by the ATPase synthase using a proton or sodium gradient generated across a lipid membrane. We present evidence that a modified electrode surface integrating a NiFeSe hydrogenase and a F1F0‐ATPase in a lipid membrane can couple the electrochemical oxidation of H2 to the synthesis of ATP. This electrode‐assisted conversion of H2 gas into ATP could serve to generate this biochemical fuel locally when required in biomedical devices or enzymatic synthesis of valuable products. |
| Keywords: | bioelectrochemistry biophysics immobilization membrane proteins proton transport |