|
|||||
|
|
High‐Throughput Measurement of Binding Kinetics by mRNA Display and Next‐Generation Sequencing |
|
| Authors: | Dr. Farzad Jalali‐Yazdi Lan Huong Lai Prof. Terry T. Takahashi Prof. Richard W. Roberts |
| Affiliation: | 1. Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles, CA, USA;2. Department of Chemistry, University of Southern California, Los Angeles, CA, USA;3. Department of Molecular Computational Biology, USC Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA, USA |
| Abstract: | There is great demand for high‐throughput methods to characterize ligand affinity. By combining mRNA display with next‐generation sequencing, we determined the kinetic on‐ and off‐rates for over twenty thousand ligands without the need for synthesis or purification of individual members. Our results are reproducible and as accurate as those obtained with other methods of affinity measurement. |
| Keywords: | affinity measurements dissociation constants high-throughput screenings kinetics protein– protein interactions |