基于荧光共振能量转移的核酸适体传感器研究

基于荧光共振能量转移的原理,以修饰于核酸适体上的FAM作为能量供体,以氧化石墨烯作为能量受体,构建了荧光适体传感器,分别对不同浓度的胰岛素和多巴胺进行检测.结果表明,胰岛素的线性检测范围为0.05~10μmol/L,多巴胺的线性检测范围为1~500μmol/L,当胰岛素和多巴胺检测浓度相同时,胰岛素检测信号远强于多巴胺.对胰岛素和多巴胺分别进行特异性实验,发现该传感器对胰岛素和多巴胺有较强的特异性.说明基于荧光共振能量转移的核酸适体传感器不仅可实现多种物质的微量检测,还具有较强的选择性,在生物和医药检测领域应用前景广阔.

Study of the Fluorescent Aptasensor Based on Fluorescence Resonance Energy Transfer

One fluorescent aptasensor based on the fluorescence resonance energy transfer was constructed and used to detect insulin and dopamine.Aptamer labeled FAM (aptamer-FAM) was in charge of energy donor,while graphene oxide served as an energy acceptor.The results show that the linear detection range of insulin is from 0.05 μmol/L to 10 μmol/L and dopamine is from 1 μmol/L to 500 μmol/L,meanwhile the signal intensity of insulin is stronger than dopamine at the same concentration.The specificity experiments for insulin and dopamine have indicated that this fluorescent aptasensor have a good specificity.Due to its detection for different targets and high selectivity,this fluorescent aptasensor offers a promising prospect in the detection field of biological and medical.